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. The quantity of affinity-purified LSM-interacting elements was unexpectedly small, in all probability as a result of technical complications, which accompany the purification of complexes from plant materials. Just the addition of a cross-linking move enhanced the process’s performance, enabling for the isolation and identification of added proteins that interact with the LSM ring. The existence and performance of two LSM complexes was corroborated by their particular interactions which has a independent list of proteins, that happen to be either involved with pre-mRNA splicing or mRNA deadenylation, decapping and decay in the case of AtLSM8 and AtLSM1 complexes, respectively.
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